In-vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

نویسندگان

  • Amir Sahraroo
  • Mesbah Babalar
  • Mohammad Hossein Mirjalili
  • Mohammad Reza Fattahi Moghaddam
  • Samad Nejad Ebrahimi
چکیده

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL(-1)). B5 medium supplemented with 1.0 mgL(-1) IBA plus 5.0 mgL(-1) BAP and MS medium fortified with 2.0 mgL(-1) IBA and 2.0 mgL(-1) BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL(-1) IBA plus 5.0 mgL(-1) BAP and MS medium fortified with 1.0 mgL(-1) IBA plus 1.0 mgL(-1) KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.

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منابع مشابه

In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

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In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

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Production of Rosmarinic Acid in Echium amoenum Fisch. and C.A. Mey. Cell Cultures

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Production of Rosmarinic Acid in Echium amoenum Fisch. and C.A. Mey. Cell Cultures

Echium amoenum Fisch. and C.A. Mey. (Boraginaceae) is a very popular medicinal plant which is used as a tonic, tranquillizer, diaphoretic, cough remedy, sore throat and pneumonia in Iran’s traditional medicine. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, callus culture of E. amoenum and its major secondary metabolite were investi...

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Callus culture of Echium amoenum Fisch. & Mey. and its major secondary metabolite

Echium amoenum Fisch. & Mey. (Boraginaceae) is a very popular medicinal plant that is used as a tonic, tranquillizer, diaphoretic, and a remedy for cough, sore throat and pneumonia in traditional medicine of Iran. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, we investigated callus culture of E. amoenum and the major secondary meta...

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عنوان ژورنال:

دوره 13  شماره 

صفحات  -

تاریخ انتشار 2014